Recombinant ZIKV (strain Zika SPH2015) Envelope protein (aa 291-696, His Tag)
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Viral Proteins
Abbreviation:
Target Synonym: E protein;Zika virus;strain Zika SPH2015
Research Areas:
Conjugation:
Target Species: ZIKV
Expression Host: Baculovirus-Insect Cells
Application:
Fusion tag: C-His
UNIProt ID:
Accession: ALU33341.1
Background: Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Concentration:
Activity: Not validated for activity
Sequence: Ile291-Thr696
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20 mM Tris, 300 mM NaCl, pH 8.0, 10 % glycerol.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 45.6 kDa.
ObservedMW:
Product Information
Product Information
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Recombinant ZIKV (strain Zika SPH2015) Envelope protein (aa 291-696, His Tag)
Recombinant ZIKV (strain Zika SPH2015) Envelope protein (aa 291-696, His Tag)
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Viral Proteins
Abbreviation:
Target Synonym: E protein;Zika virus;strain Zika SPH2015
Research Areas:
Conjugation:
Target Species: ZIKV
Expression Host: Baculovirus-Insect Cells
Application:
Fusion tag: C-His
UNIProt ID:
Accession: ALU33341.1
Background: Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Concentration:
Activity: Not validated for activity
Sequence: Ile291-Thr696
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20 mM Tris, 300 mM NaCl, pH 8.0, 10 % glycerol.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 45.6 kDa.
ObservedMW:
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Viral Proteins
Abbreviation:
Target Synonym: E protein;Zika virus;strain Zika SPH2015
Research Areas:
Conjugation:
Target Species: ZIKV
Expression Host: Baculovirus-Insect Cells
Application:
Fusion tag: C-His
UNIProt ID:
Accession: ALU33341.1
Background: Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Concentration:
Activity: Not validated for activity
Sequence: Ile291-Thr696
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20 mM Tris, 300 mM NaCl, pH 8.0, 10 % glycerol.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 45.6 kDa.
ObservedMW:
