Recombinant Mouse PTK6/Brk Protein (His & GST Tag)
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: BRK;Sik;Tksk;tks
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion tag: N-His-GST
UNIProt ID: Q64434
Accession: Q64434
Background: Tyrosine kinase (PTKs) is a protein that carry out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demenstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase(PTKs)-6/ BRK is a cytoplasmic non-receptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase(PTKs)-6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase(PTKs)-6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis.
Concentration:
Activity: The specific activity was determined to be 5 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate.
Sequence: Met1-Val451
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 79.8 kDa
ObservedMW: 66 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Recombinant Mouse PTK6/Brk Protein (His & GST Tag)
Recombinant Mouse PTK6/Brk Protein (His & GST Tag)
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: BRK;Sik;Tksk;tks
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion tag: N-His-GST
UNIProt ID: Q64434
Accession: Q64434
Background: Tyrosine kinase (PTKs) is a protein that carry out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demenstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase(PTKs)-6/ BRK is a cytoplasmic non-receptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase(PTKs)-6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase(PTKs)-6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis.
Concentration:
Activity: The specific activity was determined to be 5 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate.
Sequence: Met1-Val451
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 79.8 kDa
ObservedMW: 66 kDa
Original: $10,181,509.16
-70%$10,181,509.16
$3,054,452.75Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: BRK;Sik;Tksk;tks
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion tag: N-His-GST
UNIProt ID: Q64434
Accession: Q64434
Background: Tyrosine kinase (PTKs) is a protein that carry out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demenstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase(PTKs)-6/ BRK is a cytoplasmic non-receptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase(PTKs)-6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase(PTKs)-6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis.
Concentration:
Activity: The specific activity was determined to be 5 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate.
Sequence: Met1-Val451
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 79.8 kDa
ObservedMW: 66 kDa
