Recombinant Mouse METAP2 Protein (His Tag)
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: 4930584B20Rik;A930035J23Rik;AI047573;AL024412;AU014659;Amp2;Mnpep;p67;p67eIF2
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion tag: C-His
UNIProt ID: O08663
Accession: O08663
Background: METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.
Concentration:
Activity: Measured by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 1180-SE). The specific activity is > 15 pmol/min/¦Ìg
Sequence: Ala 2-Tyr 478
Purity: > 88 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 54.3 kDa
ObservedMW: 60 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Recombinant Mouse METAP2 Protein (His Tag)
Recombinant Mouse METAP2 Protein (His Tag)
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: 4930584B20Rik;A930035J23Rik;AI047573;AL024412;AU014659;Amp2;Mnpep;p67;p67eIF2
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion tag: C-His
UNIProt ID: O08663
Accession: O08663
Background: METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.
Concentration:
Activity: Measured by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 1180-SE). The specific activity is > 15 pmol/min/¦Ìg
Sequence: Ala 2-Tyr 478
Purity: > 88 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 54.3 kDa
ObservedMW: 60 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: 4930584B20Rik;A930035J23Rik;AI047573;AL024412;AU014659;Amp2;Mnpep;p67;p67eIF2
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion tag: C-His
UNIProt ID: O08663
Accession: O08663
Background: METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.
Concentration:
Activity: Measured by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 1180-SE). The specific activity is > 15 pmol/min/¦Ìg
Sequence: Ala 2-Tyr 478
Purity: > 88 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 54.3 kDa
ObservedMW: 60 kDa
