Recombinant Mouse EPO Receptor/EPOR Protein (His Tag)
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: Epor
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: HEK293 Cells
Application: Cell culture;ELISA
Fusion tag: C-His
UNIProt ID: P14753
Accession: NP_034279.3
Background: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.
Concentration:
Activity: 1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO. 2. Immobilized mouse EPOR-His at 10μg/mL (100μL/well) can bind biotinylated mouse EPO-His. The EC50 of biotinylated mouse EPO-His is 34. 5-80.6ng/mL.
Sequence: Met 1-Pro 249
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 26.2 kDa
ObservedMW: 30-35 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Recombinant Mouse EPO Receptor/EPOR Protein (His Tag)
Recombinant Mouse EPO Receptor/EPOR Protein (His Tag)
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: Epor
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: HEK293 Cells
Application: Cell culture;ELISA
Fusion tag: C-His
UNIProt ID: P14753
Accession: NP_034279.3
Background: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.
Concentration:
Activity: 1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO. 2. Immobilized mouse EPOR-His at 10μg/mL (100μL/well) can bind biotinylated mouse EPO-His. The EC50 of biotinylated mouse EPO-His is 34. 5-80.6ng/mL.
Sequence: Met 1-Pro 249
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 26.2 kDa
ObservedMW: 30-35 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: Epor
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: HEK293 Cells
Application: Cell culture;ELISA
Fusion tag: C-His
UNIProt ID: P14753
Accession: NP_034279.3
Background: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.
Concentration:
Activity: 1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO. 2. Immobilized mouse EPOR-His at 10μg/mL (100μL/well) can bind biotinylated mouse EPO-His. The EC50 of biotinylated mouse EPO-His is 34. 5-80.6ng/mL.
Sequence: Met 1-Pro 249
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 26.2 kDa
ObservedMW: 30-35 kDa
