Recombinant Mouse Carboxypeptidase B1/CPB1 Protein (His Tag)
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: 0910001A18Rik;1810063F02Rik;2210008M23Rik;AI504870
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: HEK293 Cells
Application: Enzyme
Fusion tag: C-His
UNIProt ID: B2RS76
Accession: NP_083982.1
Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Concentration:
Activity: Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is > 5, 000 pmoles/min/μg.
Sequence: Met 1-Tyr 415
Purity: > 94 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.5 kDa
ObservedMW: 43-45 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Recombinant Mouse Carboxypeptidase B1/CPB1 Protein (His Tag)
Recombinant Mouse Carboxypeptidase B1/CPB1 Protein (His Tag)
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: 0910001A18Rik;1810063F02Rik;2210008M23Rik;AI504870
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: HEK293 Cells
Application: Enzyme
Fusion tag: C-His
UNIProt ID: B2RS76
Accession: NP_083982.1
Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Concentration:
Activity: Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is > 5, 000 pmoles/min/μg.
Sequence: Met 1-Tyr 415
Purity: > 94 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.5 kDa
ObservedMW: 43-45 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: 0910001A18Rik;1810063F02Rik;2210008M23Rik;AI504870
Research Areas:
Conjugation:
Target Species: Mouse
Expression Host: HEK293 Cells
Application: Enzyme
Fusion tag: C-His
UNIProt ID: B2RS76
Accession: NP_083982.1
Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Concentration:
Activity: Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is > 5, 000 pmoles/min/μg.
Sequence: Met 1-Tyr 415
Purity: > 94 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.5 kDa
ObservedMW: 43-45 kDa
