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Recombinant Lactobacillus reuteri Elongation factor Tu (tuf)

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Recombinant Lactobacillus reuteri Elongation factor Tu (tuf)

Recombinant Lactobacillus reuteri Elongation factor Tu (tuf)

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Epigenetics and Nuclear Signaling

Uniprot ID: A5VJ92

Gene Names: tuf

Alternative Name(s): (EF-Tu)

Abbreviation: Recombinant Lactobacillus reuteri Elongation factor Tu protein

Organism: Lactobacillus reuteri (strain DSM 20016)

Source: E.coli

Expression Region: 1-396aa

Protein Length: Full Length

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein Sequence: MAEKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLAAKGLAKAEDYADIDAAPEEKERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVQYIVVFLNKTDLVDDDELVDLVEMEVRDLLSEYDFPGDDVPVVRGSALKALEGDPEQEKVILHLMDVIDDYIPTPKRPTDKPFMMPVEDVFTITGRGTVASGRIDRGTVKVGDEVEIVGLTEDVLKSTVTGLEMFHKTLDLGEAGDNVGVLLRGISHDQIQRGQVLAEPGSIQTHKNFKGEVYVMTKEEGGRHTPFFSNYRPQFYFHTTDVTGTIELPDGVEMVMPGDNVTFTVNLQKPVALEKGLKFTIREGGHTVGAGVVSDILD

MW: 50.9 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Reference:

Function:

$898.01
Recombinant Lactobacillus reuteri Elongation factor Tu (tuf)
$898.01

Product Information

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Description

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Epigenetics and Nuclear Signaling

Uniprot ID: A5VJ92

Gene Names: tuf

Alternative Name(s): (EF-Tu)

Abbreviation: Recombinant Lactobacillus reuteri Elongation factor Tu protein

Organism: Lactobacillus reuteri (strain DSM 20016)

Source: E.coli

Expression Region: 1-396aa

Protein Length: Full Length

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein Sequence: MAEKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLAAKGLAKAEDYADIDAAPEEKERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVQYIVVFLNKTDLVDDDELVDLVEMEVRDLLSEYDFPGDDVPVVRGSALKALEGDPEQEKVILHLMDVIDDYIPTPKRPTDKPFMMPVEDVFTITGRGTVASGRIDRGTVKVGDEVEIVGLTEDVLKSTVTGLEMFHKTLDLGEAGDNVGVLLRGISHDQIQRGQVLAEPGSIQTHKNFKGEVYVMTKEEGGRHTPFFSNYRPQFYFHTTDVTGTIELPDGVEMVMPGDNVTFTVNLQKPVALEKGLKFTIREGGHTVGAGVVSDILD

MW: 50.9 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Reference:

Function: