Recombinant Human WDYHV1/NTAQ1 Protein (GST Tag)
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: WDYHV1;NTAQ1
Target Synonym: C8orf32;N-terminal Gln amidase;NTAQ1;Nt(Q)-amidase;Protein N-terminal glutamine amidohydrolase;Protein NH2-terminal glutamine deamidase;WDYHV1
Research Areas: Cell biology
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: N-GST
UNIProt ID: Q96HA8
Accession: AAH08781.1
Background: Human protein N-terminal glutamine amidohydrolase (WDYHV1) is an enzyme that in humans is encoded by the WDYHV1 gene, belongs to the NTAQ1 family.WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, which is an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. However,it does not act on substrates with internal or C-terminal glutamine andnon-glutamine residues in any position. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
Concentration:
Activity: Not validated for activity
Sequence: Met 1-Cys205
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of PBS,100mM GSH,1% TritonX-100,15% Glycerol,pH7.4.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 49.8 kDa
ObservedMW: 45-50 kDa
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Recombinant Human WDYHV1/NTAQ1 Protein (GST Tag)
Recombinant Human WDYHV1/NTAQ1 Protein (GST Tag)
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: WDYHV1;NTAQ1
Target Synonym: C8orf32;N-terminal Gln amidase;NTAQ1;Nt(Q)-amidase;Protein N-terminal glutamine amidohydrolase;Protein NH2-terminal glutamine deamidase;WDYHV1
Research Areas: Cell biology
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: N-GST
UNIProt ID: Q96HA8
Accession: AAH08781.1
Background: Human protein N-terminal glutamine amidohydrolase (WDYHV1) is an enzyme that in humans is encoded by the WDYHV1 gene, belongs to the NTAQ1 family.WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, which is an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. However,it does not act on substrates with internal or C-terminal glutamine andnon-glutamine residues in any position. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
Concentration:
Activity: Not validated for activity
Sequence: Met 1-Cys205
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of PBS,100mM GSH,1% TritonX-100,15% Glycerol,pH7.4.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 49.8 kDa
ObservedMW: 45-50 kDa
Original: $178.22
-70%$178.22
$53.47Product Information
Product Information
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Description
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: WDYHV1;NTAQ1
Target Synonym: C8orf32;N-terminal Gln amidase;NTAQ1;Nt(Q)-amidase;Protein N-terminal glutamine amidohydrolase;Protein NH2-terminal glutamine deamidase;WDYHV1
Research Areas: Cell biology
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: N-GST
UNIProt ID: Q96HA8
Accession: AAH08781.1
Background: Human protein N-terminal glutamine amidohydrolase (WDYHV1) is an enzyme that in humans is encoded by the WDYHV1 gene, belongs to the NTAQ1 family.WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, which is an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. However,it does not act on substrates with internal or C-terminal glutamine andnon-glutamine residues in any position. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
Concentration:
Activity: Not validated for activity
Sequence: Met 1-Cys205
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of PBS,100mM GSH,1% TritonX-100,15% Glycerol,pH7.4.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 49.8 kDa
ObservedMW: 45-50 kDa
