Recombinant Human SMAD3 Protein (His & Flag Tag)
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: SMAD3
Target Synonym: JV15-2;MAD homolog 3;MADH3;Mad3;Mothers against DPP homolog 3;Mothers against decapentaplegic homolog 3;SMAD 3;SMAD family member 3;SMAD3;Smad3;hMAD-3;hSMAD3
Research Areas: Signal Transduction;Cancer;epigenetics and nuclear signal;metabolism;Stem cells
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: N-His-Flag
UNIProt ID: P84022
Accession: P84022
Background: Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.
Concentration:
Activity: Not validated for activity
Sequence: Ser2-Ser425
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 10% Glycerol, 2mM EDTA, pH 8.0.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 50.5 kDa
ObservedMW: 50-60 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Recombinant Human SMAD3 Protein (His & Flag Tag)
Recombinant Human SMAD3 Protein (His & Flag Tag)
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: SMAD3
Target Synonym: JV15-2;MAD homolog 3;MADH3;Mad3;Mothers against DPP homolog 3;Mothers against decapentaplegic homolog 3;SMAD 3;SMAD family member 3;SMAD3;Smad3;hMAD-3;hSMAD3
Research Areas: Signal Transduction;Cancer;epigenetics and nuclear signal;metabolism;Stem cells
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: N-His-Flag
UNIProt ID: P84022
Accession: P84022
Background: Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.
Concentration:
Activity: Not validated for activity
Sequence: Ser2-Ser425
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 10% Glycerol, 2mM EDTA, pH 8.0.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 50.5 kDa
ObservedMW: 50-60 kDa
Original: $3,475,958.97
-70%$3,475,958.97
$1,042,787.69Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: SMAD3
Target Synonym: JV15-2;MAD homolog 3;MADH3;Mad3;Mothers against DPP homolog 3;Mothers against decapentaplegic homolog 3;SMAD 3;SMAD family member 3;SMAD3;Smad3;hMAD-3;hSMAD3
Research Areas: Signal Transduction;Cancer;epigenetics and nuclear signal;metabolism;Stem cells
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: N-His-Flag
UNIProt ID: P84022
Accession: P84022
Background: Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.
Concentration:
Activity: Not validated for activity
Sequence: Ser2-Ser425
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 10% Glycerol, 2mM EDTA, pH 8.0.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 50.5 kDa
ObservedMW: 50-60 kDa
