Recombinant Human R-Spondin 1/RSPO1 Protein (aa 1-146, His Tag)
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: CRISTIN3;FLJ40906;R-spondin;R-spondin1;RP11-566C13.1;RP23-325M14.2;RSPO Rspo1;RSPO1;Roof plate-specific spondin-1;Rspondin
Research Areas: Developmental Biology;Stem cells
Conjugation:
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion tag: C-His
UNIProt ID: Q2MKA7
Accession: NP_001033722.1
Background: RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.
Concentration:
Activity: Measured by its ability to bind recombinant mouse CD36 in a functional ELISA.
Sequence: Met 1-Ala 146
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 14 kDa
ObservedMW: 23 kDa
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Product Information
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Shipping & Returns
Recombinant Human R-Spondin 1/RSPO1 Protein (aa 1-146, His Tag)
Recombinant Human R-Spondin 1/RSPO1 Protein (aa 1-146, His Tag)
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: CRISTIN3;FLJ40906;R-spondin;R-spondin1;RP11-566C13.1;RP23-325M14.2;RSPO Rspo1;RSPO1;Roof plate-specific spondin-1;Rspondin
Research Areas: Developmental Biology;Stem cells
Conjugation:
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion tag: C-His
UNIProt ID: Q2MKA7
Accession: NP_001033722.1
Background: RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.
Concentration:
Activity: Measured by its ability to bind recombinant mouse CD36 in a functional ELISA.
Sequence: Met 1-Ala 146
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 14 kDa
ObservedMW: 23 kDa
Original: $11,937,241.61
-70%$11,937,241.61
$3,581,172.48Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: CRISTIN3;FLJ40906;R-spondin;R-spondin1;RP11-566C13.1;RP23-325M14.2;RSPO Rspo1;RSPO1;Roof plate-specific spondin-1;Rspondin
Research Areas: Developmental Biology;Stem cells
Conjugation:
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion tag: C-His
UNIProt ID: Q2MKA7
Accession: NP_001033722.1
Background: RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.
Concentration:
Activity: Measured by its ability to bind recombinant mouse CD36 in a functional ELISA.
Sequence: Met 1-Ala 146
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 14 kDa
ObservedMW: 23 kDa
