Recombinant Human LIF Protein (E.coli)
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: LIF
Target Synonym: CDF;D Factor;DIA;Differentiation-Stimulating Factor;Emfilermin;HILDA;LIF;Leukemia Inhibitory Factor;MLPLI;Melanoma-Derived LPL Inhibitor
Research Areas: Cell biology;Neuroscience;Cancer;epigenetics and nuclear signal;immunology
Conjugation:
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion tag: N-His
UNIProt ID: P15018
Accession: P15018
Background: Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation; control of stem cell pluripotency; bone and fat metabolism; mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
Concentration:
Activity: Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <0.2 ng/mL.
Sequence: Ser23-Phe202
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
Calculated MW: 20.5 kDa
ObservedMW: 18 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Recombinant Human LIF Protein (E.coli)
Recombinant Human LIF Protein (E.coli)
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: LIF
Target Synonym: CDF;D Factor;DIA;Differentiation-Stimulating Factor;Emfilermin;HILDA;LIF;Leukemia Inhibitory Factor;MLPLI;Melanoma-Derived LPL Inhibitor
Research Areas: Cell biology;Neuroscience;Cancer;epigenetics and nuclear signal;immunology
Conjugation:
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion tag: N-His
UNIProt ID: P15018
Accession: P15018
Background: Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation; control of stem cell pluripotency; bone and fat metabolism; mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
Concentration:
Activity: Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <0.2 ng/mL.
Sequence: Ser23-Phe202
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
Calculated MW: 20.5 kDa
ObservedMW: 18 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 100μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: LIF
Target Synonym: CDF;D Factor;DIA;Differentiation-Stimulating Factor;Emfilermin;HILDA;LIF;Leukemia Inhibitory Factor;MLPLI;Melanoma-Derived LPL Inhibitor
Research Areas: Cell biology;Neuroscience;Cancer;epigenetics and nuclear signal;immunology
Conjugation:
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion tag: N-His
UNIProt ID: P15018
Accession: P15018
Background: Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation; control of stem cell pluripotency; bone and fat metabolism; mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
Concentration:
Activity: Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <0.2 ng/mL.
Sequence: Ser23-Phe202
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
Calculated MW: 20.5 kDa
ObservedMW: 18 kDa
