Recombinant Human ISG15/G1P2 Protein
Size: 1mg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: G1P2;IP17;ISG15;Interferon-Induced 15 kDa Protein;Interferon-Induced 17 kDa Protein;UCRP;Ubiquitin Cross-Reactive Protein;Ubiquitin-Like Protein ISG15;hUCRP
Research Areas: Cell Biology,Epigenetics and Nuclear Signaling
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: None
UNIProt ID: P05161
Accession: AAH09507.1
Background: Interferon-induced 17 kDa protein (ISG15), a 15-kDa protein of unique primary amino acid sequence, functions intracellularly as an ubiquitin homologue and a cytokine that induces production of IFN-gamma and augments NK / lymphokine-activated killer cell proliferation and function. ISG15 is secreted from monocytes and lymphocytes. ISG15 is an ubiquitin-like molecule that is strongly upregulated by type I interferons as a primary response to diverse microbial and cellular stress stimuli. Alterations in the ISG15 signalling pathway have also been found in several human tumour entities. In addition to being stimulated by type I interferon, expression of ISG15 is greatly induced by viral or bacterial infection through the Janus kinase / signal transducer and activator of transcription (Jak / STAT) signalling pathway. After induction, ISG15 is secreted by monocytes, B- and T-lymphocytes and fibroblasts. We demonstrate the novel way in which the function of the ISG15 protein is inhibited by influenza B virus, which strongly induces the ISG15 protein: a specific region of the influenza B virus NS1 protein, which includes part of its effector domain, blocks the covalent linkage of ISG15 to its target proteins both in vitro and in infected cells.
Concentration:
Activity: Not validated for activity
Sequence: Met 1-Ser 165
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50mM Tris, pH 8.0
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 17.9 kDa
ObservedMW: 16 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Recombinant Human ISG15/G1P2 Protein
Recombinant Human ISG15/G1P2 Protein
Size: 1mg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: G1P2;IP17;ISG15;Interferon-Induced 15 kDa Protein;Interferon-Induced 17 kDa Protein;UCRP;Ubiquitin Cross-Reactive Protein;Ubiquitin-Like Protein ISG15;hUCRP
Research Areas: Cell Biology,Epigenetics and Nuclear Signaling
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: None
UNIProt ID: P05161
Accession: AAH09507.1
Background: Interferon-induced 17 kDa protein (ISG15), a 15-kDa protein of unique primary amino acid sequence, functions intracellularly as an ubiquitin homologue and a cytokine that induces production of IFN-gamma and augments NK / lymphokine-activated killer cell proliferation and function. ISG15 is secreted from monocytes and lymphocytes. ISG15 is an ubiquitin-like molecule that is strongly upregulated by type I interferons as a primary response to diverse microbial and cellular stress stimuli. Alterations in the ISG15 signalling pathway have also been found in several human tumour entities. In addition to being stimulated by type I interferon, expression of ISG15 is greatly induced by viral or bacterial infection through the Janus kinase / signal transducer and activator of transcription (Jak / STAT) signalling pathway. After induction, ISG15 is secreted by monocytes, B- and T-lymphocytes and fibroblasts. We demonstrate the novel way in which the function of the ISG15 protein is inhibited by influenza B virus, which strongly induces the ISG15 protein: a specific region of the influenza B virus NS1 protein, which includes part of its effector domain, blocks the covalent linkage of ISG15 to its target proteins both in vitro and in infected cells.
Concentration:
Activity: Not validated for activity
Sequence: Met 1-Ser 165
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50mM Tris, pH 8.0
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 17.9 kDa
ObservedMW: 16 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 1mg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: G1P2;IP17;ISG15;Interferon-Induced 15 kDa Protein;Interferon-Induced 17 kDa Protein;UCRP;Ubiquitin Cross-Reactive Protein;Ubiquitin-Like Protein ISG15;hUCRP
Research Areas: Cell Biology,Epigenetics and Nuclear Signaling
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: None
UNIProt ID: P05161
Accession: AAH09507.1
Background: Interferon-induced 17 kDa protein (ISG15), a 15-kDa protein of unique primary amino acid sequence, functions intracellularly as an ubiquitin homologue and a cytokine that induces production of IFN-gamma and augments NK / lymphokine-activated killer cell proliferation and function. ISG15 is secreted from monocytes and lymphocytes. ISG15 is an ubiquitin-like molecule that is strongly upregulated by type I interferons as a primary response to diverse microbial and cellular stress stimuli. Alterations in the ISG15 signalling pathway have also been found in several human tumour entities. In addition to being stimulated by type I interferon, expression of ISG15 is greatly induced by viral or bacterial infection through the Janus kinase / signal transducer and activator of transcription (Jak / STAT) signalling pathway. After induction, ISG15 is secreted by monocytes, B- and T-lymphocytes and fibroblasts. We demonstrate the novel way in which the function of the ISG15 protein is inhibited by influenza B virus, which strongly induces the ISG15 protein: a specific region of the influenza B virus NS1 protein, which includes part of its effector domain, blocks the covalent linkage of ISG15 to its target proteins both in vitro and in infected cells.
Concentration:
Activity: Not validated for activity
Sequence: Met 1-Ser 165
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50mM Tris, pH 8.0
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 17.9 kDa
ObservedMW: 16 kDa
