Recombinant Human EphB1/EPHT2 Protein (aa 1-540, His Tag)
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: EHK-3;EHK3;EK11;EPH Homology Kinase 3;EPH-Like Kinase 11;EPHA7;Ephrin Type-A Receptor 7;HEK11;hEK11
Research Areas: Cardiovascular,Neuroscience,Signal Transduction,Stem Cells,Cancer
Conjugation:
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion tag: C-His
UNIProt ID: P54762
Accession: P54762-1
Background: Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
Concentration:
Activity: Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB1-Fc2h with a linear ranger of 3. 125-200 ng/mL. Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB2-Fch with a linear ranger of 0.3125-20 ng/mL.
Sequence: Met 1-Pro 540
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4, 5% glycerol
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 60.0 kDa
ObservedMW: 60 kDa
Product Information
Product Information
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Shipping & Returns
Recombinant Human EphB1/EPHT2 Protein (aa 1-540, His Tag)
Recombinant Human EphB1/EPHT2 Protein (aa 1-540, His Tag)
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: EHK-3;EHK3;EK11;EPH Homology Kinase 3;EPH-Like Kinase 11;EPHA7;Ephrin Type-A Receptor 7;HEK11;hEK11
Research Areas: Cardiovascular,Neuroscience,Signal Transduction,Stem Cells,Cancer
Conjugation:
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion tag: C-His
UNIProt ID: P54762
Accession: P54762-1
Background: Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
Concentration:
Activity: Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB1-Fc2h with a linear ranger of 3. 125-200 ng/mL. Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB2-Fch with a linear ranger of 0.3125-20 ng/mL.
Sequence: Met 1-Pro 540
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4, 5% glycerol
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 60.0 kDa
ObservedMW: 60 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation:
Target Synonym: EHK-3;EHK3;EK11;EPH Homology Kinase 3;EPH-Like Kinase 11;EPHA7;Ephrin Type-A Receptor 7;HEK11;hEK11
Research Areas: Cardiovascular,Neuroscience,Signal Transduction,Stem Cells,Cancer
Conjugation:
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion tag: C-His
UNIProt ID: P54762
Accession: P54762-1
Background: Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
Concentration:
Activity: Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB1-Fc2h with a linear ranger of 3. 125-200 ng/mL. Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB2-Fch with a linear ranger of 0.3125-20 ng/mL.
Sequence: Met 1-Pro 540
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4, 5% glycerol
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 60.0 kDa
ObservedMW: 60 kDa
