Recombinant Human CD172a/SIRPA Protein (His Tag)
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Immune Checkpoint Proteins
Abbreviation: CD172a;SIRPA
Target Synonym: Bit;Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs;CD172 Antigen-Like Family Member A;Inhibitory Feceptor SHPS-1;Macrophage Fusion Receptor;MyD-1 Antigen;SHP Substrate 1;SHPS-1;Signa;Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1
Research Areas: Neuroscience,Cardiovascular
Conjugation:
Target Species: Human
Expression Host: HEK293 Cells
Application: BLI
Fusion tag: C-His
UNIProt ID: P78324
Accession: CAA71403.1
Background: Signal Regulatory Protein α (SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation
Concentration:
Activity: Loaded Anti-Human SIRPA mAb-Fc on Protein A Biosensor, can bind Human SIRPA-His with an affinity constant of 10.1 nM as determined in BLI assay.
Sequence: Glu31-Arg370
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 38.3 kDa
ObservedMW: 45-65 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Recombinant Human CD172a/SIRPA Protein (His Tag)
Recombinant Human CD172a/SIRPA Protein (His Tag)
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Immune Checkpoint Proteins
Abbreviation: CD172a;SIRPA
Target Synonym: Bit;Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs;CD172 Antigen-Like Family Member A;Inhibitory Feceptor SHPS-1;Macrophage Fusion Receptor;MyD-1 Antigen;SHP Substrate 1;SHPS-1;Signa;Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1
Research Areas: Neuroscience,Cardiovascular
Conjugation:
Target Species: Human
Expression Host: HEK293 Cells
Application: BLI
Fusion tag: C-His
UNIProt ID: P78324
Accession: CAA71403.1
Background: Signal Regulatory Protein α (SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation
Concentration:
Activity: Loaded Anti-Human SIRPA mAb-Fc on Protein A Biosensor, can bind Human SIRPA-His with an affinity constant of 10.1 nM as determined in BLI assay.
Sequence: Glu31-Arg370
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 38.3 kDa
ObservedMW: 45-65 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 50μg
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Immune Checkpoint Proteins
Abbreviation: CD172a;SIRPA
Target Synonym: Bit;Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs;CD172 Antigen-Like Family Member A;Inhibitory Feceptor SHPS-1;Macrophage Fusion Receptor;MyD-1 Antigen;SHP Substrate 1;SHPS-1;Signa;Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1
Research Areas: Neuroscience,Cardiovascular
Conjugation:
Target Species: Human
Expression Host: HEK293 Cells
Application: BLI
Fusion tag: C-His
UNIProt ID: P78324
Accession: CAA71403.1
Background: Signal Regulatory Protein α (SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation
Concentration:
Activity: Loaded Anti-Human SIRPA mAb-Fc on Protein A Biosensor, can bind Human SIRPA-His with an affinity constant of 10.1 nM as determined in BLI assay.
Sequence: Glu31-Arg370
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 38.3 kDa
ObservedMW: 45-65 kDa
