Recombinant Human BRD4 (N-10His-Flag)
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: BRD4
Target Synonym: HUNK1;HUNK1bromodomain-containing protein 4;HUNKI;MCAP
Research Areas: Cancer;epigenetics and nuclear signal
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: N-His-Flag
UNIProt ID: O60885
Accession: O60885
Background: Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.
Concentration:
Activity: Not validated for activity
Sequence: Glu49-Glu460
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 50mM HEPES, 200mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 49.0 kDa
ObservedMW: 55-60 kDa
Product Information
Product Information
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Shipping & Returns
Recombinant Human BRD4 (N-10His-Flag)
Recombinant Human BRD4 (N-10His-Flag)
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: BRD4
Target Synonym: HUNK1;HUNK1bromodomain-containing protein 4;HUNKI;MCAP
Research Areas: Cancer;epigenetics and nuclear signal
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: N-His-Flag
UNIProt ID: O60885
Accession: O60885
Background: Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.
Concentration:
Activity: Not validated for activity
Sequence: Glu49-Glu460
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 50mM HEPES, 200mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 49.0 kDa
ObservedMW: 55-60 kDa
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
Size: 50μg
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Exp date: 12 months
Category ID_II: Recombinant Proteins
Category ID_III: Others
Abbreviation: BRD4
Target Synonym: HUNK1;HUNK1bromodomain-containing protein 4;HUNKI;MCAP
Research Areas: Cancer;epigenetics and nuclear signal
Conjugation:
Target Species: Human
Expression Host: E.coli
Application:
Fusion tag: N-His-Flag
UNIProt ID: O60885
Accession: O60885
Background: Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.
Concentration:
Activity: Not validated for activity
Sequence: Glu49-Glu460
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 50mM HEPES, 200mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 49.0 kDa
ObservedMW: 55-60 kDa
