Size: 200μL

Storage: Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping: The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.

Exp date: 12 months

Category ID_II: Primary Antibodies

Category ID_III: Acetyl/Phospho/Methyl Antibodies

Abbreviation: AMPKα1/2 (phospho Thr183/172)

Target Synonym: PRKAA1;AMPK1;5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;Acetyl-CoA carboxylase kinase;ACACA kinase;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;Tau-protein kinase PRKAA1;PRKAA2;AMPK;

Research Areas: Cancer;Cardiovascular;Metabolism;Neuroscience;Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species reactivity: Human;Mouse;Rat;Monkey

Application: WB;IHC-p;IF

Isotype: IgG

Clonality: Polyclonal

Clone NO.:

UNIProt ID: Q13131,P54646

Accession:

Background: AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.

Concentration: 1 mg/mL

Immunogen: Synthesized peptide derived from human AMPKα1/2 around the phosphorylation site of Thr183/172

Buffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Purification method: Affinity purification

Dilution: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:50-1:200

Calculated MW: 62kDa

ObservedMW: 63kDa