Size: 200μL

Storage: Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping: The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.

Exp date: 12 months

Category ID_II: Primary Antibodies

Category ID_III: Polyclonal Antibodies

Abbreviation: ADO

Target Synonym: 2 aminoethanethiol dioxygenase;2-aminoethanethiol dioxygenase;ADO 2 aminoethanethiol (cysteamine) dioxygenase;ADO;AEDO;C10orf22;Chromosome 10 open reasing frame 22;cysteamine (2-aminoethanethiol) dioxygenase (ADO);Cysteamine dioxygenase;DKFZp564C046;FLJ14547

Research Areas: Metabolism;Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species reactivity: Human;Mouse

Application: WB;IHC

Isotype: IgG

Clonality: Polyclonal

Clone NO.:

UNIProt ID: Q96SZ5

Accession:

Background: Human thiol dioxygenases include cysteine dioxygenase (CDO, MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO, EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Concentration: 0.3 mg/mL

Immunogen: Synthetic peptide of human ADO

Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4

Purification method: Affinity purification

Dilution: WB 1:1000-1:5000;IHC 1:25-1:100

Calculated MW: 30kDa

ObservedMW: