TransNGS^® Single Cell Full Length cDNA Synthesis & Amplification Kit is a reagent kit capable of obtaining amplified full-length cDNA from single cell. It is suitable for amplifying and constructing cDNA libraries from 1-500 cells or 10 pg-10 ng total RNA. The kit uses Single Cell Oligo (dT) as the reverse transcription primer and employs a reverse transcriptase with high synthesis efficiency and template-switching activity to add a special sequence to the 3' end of the cDNA, thereby obtaining full-length cDNA products. The kit is compatible with various cell types and tissue types, suitable for cell materials with varying RNA content. Typically, a single-cell library can yield 10-60 ng of amplified cDNA products.

Features

• Strong compatibility with different cell types, suitable for cells with low RNA content (such as immune cells).

• Strong compatibility with different tissue types, suitable for tissues that are difficult to dissociate (such as brain tissue).

• High yield in constructing single-cell libraries, excellent peak shapes, high efficiency in gene detection (FPKM > 1)

Storage

Single cell TSO should be stored at -70℃ for one year. The other components should be stored at -20℃ for one year

Shipping

Dry ice (-70℃)

Product Composition

Component	KC901-01 (12 rxns)	KC901-02 (24 rxns)	KC901-03 (96 rxns)
Single Cell Lysis Buffer	12 μl	24 μl	96 μl
Single Cell Oligo(dT)	12 μl	24 μl	96 μl
10 mM dNTPs	12 μl	24 μl	96 μl
Ribonuclease Inhibitors	5 μl	10 μl	40 μl
Single Cell RT Buffer	24 μl	48 μl	192 μl
DTT	12 μl	24 μl	96 μl
Single Cell TSO	6 μl	12 μl	48 μl
Single Cell RT Enhancer	6 μl	12 μl	48 μl
Single Cell Reverse Transcriptase	12 μl	24 μl	96 μl
2×Single Cell Amplification SuperMix	300 μl	600 μl	2.4 ml
Single Cell PCR Primer	12 μl	24 μl	96 μl
RNase-free Water	1 ml	2 ml	5 ml
Library Elution Buffer	300 μl	600 μl	2.4 ml

Next Generation Sequencing

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